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Commercially-Available Hydrolyzed Fish Collagen Peptides in Preventive Control of Rheumatoid Arthritis Using Mouse Model

 

Tin-Chen Hsu¹

Masanobu Shimokawa²

Hajime Inoue³

 

¹ Chien Cheng Trading Co., Ltd., Taipei, Taiwan. cheng181@ms4.hinet.net

^{2 3} Plastic & Reconstructive Surgery, St. Marianna University School of Medicine, Tokyo, Japan. blue_snail@hotmail.co.jp, h2inoue@marianna-u.ac.jp

 

Abstract

Oral administration of cartilage-derived native type II collagen was previously reported to ameliorate rheumatoid arthritis (RA) in animal models of joint inflammation where the therapeutic activity may be resulted from accumulation of hydrolyzed collagen around limb joint leading to induced collagen production by chondrocytes. We study the ingestion of collagen hydrolysates (CHF, hydrolyzed fish collagen peptides from RABJ Co. Ltd.) in reduction of limb swelling using a mouse model (SKG/Jcl) with spontaneous onset of RA.  CHF could permeate through synthetic skin model using cultured keratinocytes while high molecular collagen protein could not.  CHF stimulated collagen synthesis in cultured dermal fibroblast suggesting the functionality of the percutaneous absorbed CHF. The intake of CHF by fibroblasts was statistically greater than the intake of either the native collagen protein or hydrolyzed collagen peptides from other producers.  In order to determine the activity of CHF in control of RA, we administer CHF into the diet for SKG/Jcl mice that naturally develops a chronic T cell-mediated autoimmune disease mimicking RA. Oral ingestion of CHF contained the spontaneous progression of arthritis in SKG/Jcl mice, as demonstrated by both limb volume and arthritis scores suggesting that CHF could suppress or delay the onset of RA in SKG/Jcl mouse model.  Administration of a formulated recipe containing CHF and N-acetyl glucosamine showed greater suppression of RA in laminarin(adjuvant)-induced SKG/Jcl mouse suggesting a likely synergy between collagen and glucosamine. We demonstrate here that non-native hydrolyzed collagen peptides could be used in preventive control of joint inflammation caused by rheumatoid arthritis.

 

Key words: Fish collagen peptide, Rheumatoid Arthritis (RA), Anti-inflammation effect